作者：王婷，付建莉，张雪梅，赵媛，赵玉兰

单位：

1.陕西省人民医院老年医学科2.陕西省人民医院心脏外科3.陕西省人民医院超声诊断中心4.郑州大学第二附属医院心内一科

Units：

1.Department of Geriatrics, Shaanxi Provincial People's Hospital, Xi'an 710068, China 2.Department of Cardiovascular Surgery, Shaanxi Provincial People's Hospital, Xi'an 710068, China 3.Department of Ultrasonic Medicine, Shaanxi Provincial People's Hospital, Xi'an 710068, China 4.Department of Cardiology, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China

关键词：

维生素D3; 胆骨化醇; 腹主动脉缩窄; 心脏结构; 心脏功能; 大鼠;

Keywords：

Vitamin D3; Cholecalciferol; Coarctation of abdominal aorta; Cardiac structure; Cardiac function; Rats

CLC：

DOI：

10.12114/j.issn.1008-5971.2023.00.158

Funds：

陕西省重点研发计划项目（2022SF-059）

摘要：

目的 分析不同剂量维生素D3对腹主动脉压力过负荷大鼠心脏结构、功能的影响。方法 本实验时间为2021年6月。选取健康雄性Wistar大鼠60只，采用随机数字表法将其分为安慰剂组(A组)、小剂量组(B组)、中等剂量组(C组)、大剂量组(D组)，每组15只。四组大鼠均采用腹主动脉缩窄术制备腹主动脉压力过负荷模型，然后按照Sharma方法进行换算，分别给予安慰剂、小剂量维生素D3(84 U/kg)、中等剂量维生素D3(420 U/kg)、大剂量维生素D3(2 100 U/kg)进行灌胃，连续灌胃8周。观察大鼠进食及活动耐量情况。比较四组大鼠灌胃8周后超声心动图检查指标[室间隔厚度(IVST)、左心室后壁厚度(LVPWT)、左心室收缩末期内径(LVESD)、左心室舒张末期内径(LVEDD)和左心室射血分数(LVEF)]和实验室检查指标{血清钙、磷、25羟维生素D[25(OH)D]、N末端脑钠肽前体(NT-proBNP)水平}。结果 术中及术后共7只大鼠死亡，其中A组死亡2只、B组死亡2只、C组死亡1只、D组死亡2只。四组大鼠进食及活动耐量均无明显差异。灌胃8周后，B组大鼠IVST、LVPWT、LVESD、LVEDD小于A组(P<0.05);C组大鼠IVST和LVEDD大于B组，LVPWT、LVESD、LVEDD小于A组(P<0.05);D组大鼠IVST、LVPWT、LVESD、LVEDD大于A、B、C组，LVEF低于B、C组(P<0.05)。灌胃8周后，B、C、D组大鼠血清钙、25(OH)D水平高于A组，C、D组大鼠血清钙、25(OH)D水平高于B组，D组大鼠血清钙、25(OH)D水平高于C组(P<0.05)。灌胃8周后，B、C组大鼠血清NT-proBNP水平低于A、D组(P<0.05)。结论 维生素D3补充剂量与腹主动脉压力过负荷大鼠心脏结构、功能变化呈U型曲线关系，即补充小剂量维生素D3能减轻腹主动脉压力过负荷大鼠心脏结构、功能的恶化，而补充大剂量维生素D3反而促进了腹主动脉压力过负荷大鼠心脏结构、功能的恶化。

Abstract：

Objective To analyze the effects of different doses of vitamin D3 on cardiac structure and function in rats with abdominal aortic pressure overload. Methods The experiment was conducted in June 2021. Sixty healthy male Wistar rats were selected and divided into placebo group (group A) , low-dose group (group B) , medium-dose group (group C) and high-dose group (group D) by random number table method, with 15 rats in each group. The rats of four groups were used to prepare abdominal aortic pressure overload model by abdominal aortic constriction. According to the Sharma method, the rats of four groups were given placebo, low-dose vitamin D3 (84 U/kg) , medium-dose vitamin D3 (420 U/kg) , and high-dose vitamin D3 (2 100 U/kg) by gavage for 8 weeks. The feeding and activity tolerance of rats were observed. Echocardiography examination indicators [interventricular septal thickness (IVST) , left ventricular posterior wall thickness (LVPWT) , left ventricular endsystolic diameter (LVESD) , left ventricular end-diastolic diameter (LVEDD) and left ventricular ejection fraction (LVEF) ] and laboratory examination indexes {serum calcium, phosphorus, 25-hydroxyvitamin D [25 (OH) D] , N-terminal pro-brain natriuretic peptide (NT-proBNP) levels} were compared among the four groups after 8 weeks of gavage. Results A total of 7 rats died during operation and after operation, including 2 rats in group A, 2 rats in group B, 1 rat in group C and 2 rats in group D. There was no significant difference in eating and activity tolerance among the four groups. After 8 weeks of gavage, IVST, LVPWT, LVESD and LVEDD in group B were smaller than those in group A (P < 0.05) ; IVST and LVEDD in group C were larger than those in group B, and LVPWT, LVESD and LVEDD were lower than those in group A (P < 0.05) ; IVST, LVPWT, LVESD and LVEDD in group D were larger than those in groups A, B and C, and LVEF was lower than that in groups B and C (P < 0.05) . After 8 weeks of gavage, the serum calcium and 25 (OH) D levels in groups B, C and D were higher than those in group A, the serum calcium and 25 (OH) D levels in groups C and D were higher than those in group B, and the serum calcium and 25 (OH) D levels in group D were higher than those in group C (P < 0.05) . After 8 weeks of gavage, serum NT-proBNP level in groups B and C was lower than that in groups A and D (P < 0.05) . Conclusion There was a U-shaped curve relationship between vitamin D3 supplementation dose and the changes of cardiac structure and function in rats with abdominal aortic pressure overload. Supplementation of lowdose vitamin D3 can alleviate the deterioration of cardiac structure and function in abdominal aortic pressure overload rats, while supplementation of high-dose vitamin D3 promotes the deterioration of cardiac structure and function in abdominal aortic pressure overload rats.

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