作者：陈平1，苟惠2，吴文兵3，黄露雯4，任思冲5

单位：

1.629000四川省遂宁市中心医院药学部2.646000四川省泸州市，西南医科大学附属医院药学部3.646000四川省泸州市，西南医科大学基础医学院生物化学与分子生物学教研室4.629000四川省遂宁市中心医院神经内科5.610500四川省成都市，成都医学院第一附属医院肾内科

Units：

1.Department of Pharmacy, Suining Central Hospital, Suining 629000, China2.Department of Pharmacy, Southwest Medical University, Luzhou 646000, China3.Department of Biochemistry and Molecular Biology, Southwest Medical University, School of Basic Medical Sciences, Luzhou646000, China4.Department of Neurology, Suining Central Hospital, Suining 629000, China5.Department of Nephrology, the First Affiliated Hospital of Chengdu Medical College, Chengdu 610500, China

关键词：

脑膜炎，利斯特菌性；单核细胞增生李斯特菌；脑脊液；二代测序；病例报告

Keywords：

Meningitis, Listeria; Listeria monocytogenes; Cerebrospinal fluid; Next-generation sequencing; Case reports

CLC：

　R 515.2　

DOI：

10.12114/j.issn.1008-5971.2022.00.263

Funds：

国家自然科学基金青年科学基金项目（81803967）

摘要：

单核细胞增生李斯特菌脑膜炎临床少见，病死率高，传统检测方法不易获得其病原学证据，且单核细胞增生李斯特菌对头孢菌素类抗生素天然耐药，因而其早期诊断与精准治疗仍是临床面临的挑战。二代测序技术使得单核细胞增生李斯特菌脑膜炎病原菌检出率不断提高，本文报道2例通过脑脊液二代测序成功确诊单核细胞增生李斯特菌脑膜炎病例，并及时给予合理抗菌药物治疗，患者预后良好，以期为临床提供参考。

Abstract：

Listeria monocytogenes meningitis is a rare disease with high mortality. Traditional detection methodsare difficult to obtain its etiological evidence and Listeria monocytogenes are naturally resistant to cephalosporin antibiotics.Therefore, its early diagnosis and precise treatment are still clinical challenges. Next-generation sequencing technology improvesthe detection rate of Listeria monocytogenes meningitis. In order to provide clinical reference, this article reports two cases ofListeria monocytogenes meningitis who successfully detected by next-generation sequencing technology of cerebrospinal fluid,timely treated with reasonable antibiotics and had good prognosis.

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