作者：梁宇明，何燕

单位：

530021 广西壮族自治区南宁市，广西医科大学第一附属医院老年心内科；通信作者：何燕，E-mail：hyxjwxy@126.com

关键词：

高血压；骨形成蛋白和激活素的跨膜抑制剂；转化生长因子 β1；心律失常；大鼠

Keywords：

Hypertension；Bone morphogenetic protein and activin membrane-bound inhibitor；Transforminggrowth factor beta 1；Arrhythmia；Rats

CLC：

R 544.1

DOI：

DOI：10.3969/j.issn.1008-5971.2020.05.012

Funds：

国家自然科学基金资助项目（81760061）

摘要：

背景　动物实验研究表明，左心室心肌组织骨形成蛋白和激活素的跨膜抑制剂（BAMBI）表达上调可对抗转化生长因子 β1（TGF-β1）的致心肌纤维化作用，而心肌纤维化又与室性心律失常（VA）密切相关，因此推测 BAMBI 和 TGF-β1 可能参与了高血压患者 VA 的发生发展过程。目的　分析自发性高血压大鼠左心室心肌组织 BAMBI、TGF-β1 表达情况及其与 VA 易感性的关系。方法　2018 年 7 月—2019 年 7 月，选取 6 只 14 周龄雄性Wistar 大鼠作为对照组，6 只与 Wistar 大鼠同源的雄性自发性高血压大鼠作为实验组；两组大鼠均饲养至 22 周龄。比较两组大鼠 22 周龄时收缩压、心率、心脏彩超检查结果〔包括左心室舒张末期内径（LVEDD）、左心室收缩末期内径（LVESD）、左心室射血分数（LVEF）及左心室短轴缩短率（LVFS）〕、VA 诱发成功率，左心室心肌组织BAMBI、TGF-β1 mRNA及其相对表达量。结果　两组大鼠22周龄时心率和LVEDD比较，差异无统计学意义（P>0.05）；实验组大鼠 22 周龄时收缩压高于对照组，LVESD 大于对照组，LVEF 和 LVFS 低于对照组（P<0.05）。实验组大鼠22 周龄时 VA 诱发成功率高于对照组（P<0.05）。实验组大鼠 22 周龄时左心室心肌组织 BAMBI 和 TGF-β1 mRNA及其相对表达量均高于对照组（P<0.05）。结论　22 周龄自发性高血压大鼠左心室心肌组织中 BAMBI 和 TGF-β1 表达上调可导致心肌纤维化加重、左心室重构，进而增加 VA 易感性。

Abstract：

Background　Animal studies showed that，up-regulated expression of BAMBI in left ventricularmyocardium can counteract the myocardial fibrosis caused by TGF-β1，however myocardial fibrosis is closely related toventricular arrhythmia（VA），therefore it is being speculated that BAMBI and TGF-β1 may be involved in the occurrenceand development of VA in patients with hypertension. Objective　To analyze the expressions of BAMBI and TGF-β1 inleft ventricular myocardial tissue and their relations to susceptibility of VA in spontaneously hypertensive rats（SHR）.Methods　From July 2018 to July 2019，a total of six 14 week-old male Wistar rats were selected as control group，meanwhilesix 14 week-old homologous male SHR rats were selected as experiment group；rats in the two groups were raised to 22 week-old. SBP，heart rate，color Doppler echocardiography examination results（including LVEDD，LVESD，LVEF and LVFS），success rate of induction of VA，BAMBI and TGF-β1 mRNA and their relative expression quantity in left ventricular myocardialtissue were compared between the two groups at 22 weeks old. Results　There was no statistically significant difference in heartrate or LVEDD between the two groups at 22 weeks old（P>0.05）；SBP in experiment group was statistically significantlyhigher than that in control group，LVESD in experiment group was statistically significantly greater than that in control group，while LVEF and LVFS in experiment group were statistically significantly lower than those in control group（P<0.05）.Successrate of induction of VA in experiment group was statistically significantly higher than that in control group at 22 weeks old（P<0.05）.BAMBI and TGF-β1 mRNA and their relative expression quantity in left ventricular myocardial tissue inexperiment group were statistically significantly higher than those in control group at 22 weeks old，respectively（P<0.05）.Conclusion　Expressions of BAMBI and TGF-β1 in left ventricular myocardial tissue are up-regulated in 22 week-old SHR，whichmay lead to the aggravation of myocardial fibrosis and the reconstruction of left ventricle，result in increase of the susceptibility of VR.

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