作者：王玉玲1，2 ，高华 2，3 ，蒋森 2 ，李艳霞 2 ，杨新玲 2

单位：

1.830054 新疆维吾尔自治区乌鲁木齐市，新疆医科大学第一附属医院干部保健中心　；2.830063 新疆维吾尔自治区乌鲁木齐市，新疆医科大学第二附属医院神经内科　；3.830011 新疆维吾尔自治区乌鲁木齐市，新疆医科大学第五附属医院神经内科；通信作者：杨新玲，E-mail：poplar862@sohu.com

关键词：

帕金森病；大鼠；Fas/Fasl 信号通路；多巴胺能神经元

Keywords：

Parkinson's disease；Rat；Fas/Fasl；Dopaminergic neurons

CLC：

R 742.5

DOI：

DOI：10.3969/j.issn.1008-5971.2020.04.016

Funds：

国家自然科学基金资助项目（U1503222）

摘要：

背景　免疫炎症可能是导致帕金森病（PD）的因素，而 Fas/FasL 信号通路诱导的淋巴细胞凋亡与心血管疾病、自身免疫系统疾病的发生及预后均密切相关。目的　探讨 Fas/FasL 信号通路与 PD 大鼠多巴胺能神经元的相关性。方法　2017 年 10 月—2018 年 1 月，从 60 只雄性 SPF 级 SD 大鼠随机选取 10 只作为对照组，其余大鼠采用脑立体定位技术建立 PD 大鼠模型，并将建模成功的 20 只大鼠随机分为模型组与干预组，每组 10 只。对照组、模型组大鼠给予 0.9% 氯化钠溶液 2 ml 灌胃，干预组大鼠则给予美多芭溶液 2 ml 灌胃，均为 1 次 /d，连续灌胃 14 d。比较模型组与干预组大鼠干预后阿扑吗啡（APO）诱导的旋转圈数，并比较三组大鼠脑组织 Fas、FasL、酪氨酸氢化酶（TH）蛋白表达水平；TH 蛋白表达水平与 PD 大鼠 Fas、FasL 蛋白表达水平的相关性采用 Pearson 相关分析。结果　干预组大鼠干预后 APO 诱导的旋转圈数少于模型组（P<0.05）。对照组与干预组大鼠的 Fas、FasL 蛋白表达水平低于模型组，TH 蛋白表达水平高于模型组（P<0.05）；干预组大鼠的 Fas、FasL 蛋白表达水平高于对照组，TH 蛋白表达水平低于对照组（P<0.05）。Pearson 相关分析结果显示，TH 蛋白表达水平与 Fas、FasL 蛋白表达水平无直线相关性（r 值分别为 0.053、0.026，P>0.05）。结论　PD 大鼠 Fas、FasL 蛋白表达水平较高，Fas/FasL 信号通路激活，但多巴胺能神经元数量较少，且 Fas/FasL 信号通路与 PD 大鼠多巴胺能神经元无直线相关关系。

Abstract：

Background　Immune inflammation is a possible factor of Parkinson's disease，however Fas/FasLsignaling pathway-induced lymphocyte apoptosis is closely correlated with the occurrence and prognosis of cardiovasculardisease and autoimmune system disease. Objective　To investigate the correlation between Fas/FasL signaling pathway anddopaminergic neurons in rats with Parkinson's disease. Methods　From October 2017 to January 2018，10 out of 60 maleSPF SD rats were randomly selected as control group，while the other 50 rats were used to prepare for Parkinson's diseasemodels by stereotactic brain technology，after that 20 rats with successful preparation for Parkinson's disease models randomlydivided into model group（n=10）and intervention group（n=10）. Rats in control group and model group received intragastricadministration of 0.9% sodium chloride solution（2 ml per time，1 time per day）for 14 days，while rats in intervention groupreceived intragastric administration of Madopar solution（2 ml per time，1 time per day）for 14 days. APO-induced number ofrotations was compared between model group and intervention group after intervention，meanwhile protein expression levels ofFas，FasL and tyrosine hydrogenase（TH）in brain tissue were compared among the three groups after intervention；Pearsoncorrelation analysis was used to analyze the correlations of protein expression level of TH with protein expression levels of Fasand FasL. Results　APO-induced number of rotations in intervention group was statistically significantly less than that in modelgroup after intervention（P<0.05）.Protein expression levels of Fas and FasL in brain tissue in control group and interventiongroup were statistically significantly lower than those in model group after intervention，while protein expression level of TH inbrain tissue in control group and intervention group was statistically significantly higher than that in model group，respectively（P<0.05）；protein expression levels of Fas and FasL in brain tissue in intervention group were statistically significantly higherthan thsoe in control group after intervention，while protein expression level of TH in brain tissue in intervention group wasstatistically significantly lower than that in control group（P<0.05）.Pearson correlation analysis results showed that，proteinexpression level of TH was not linearly correlated protein expression level of Fas（r=0.053）or FasL（r=0.026）in rats withParkinson's disease（P>0.05）. Conclusion　Protein expression levels of Fas and FasL are significantly high and Fas/FasLsignaling pathway is activated in rats with Parkinson's disease，but the number dopaminergic neurons is relatively，moreoverFas/FasL signaling pathway is not linearly correlated the number dopaminergic neurons in rats with Parkinson's disease.

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