作者：陈国萍，刘娟利，朱晓莉，薛方苏

单位：

1.224500江苏省盐城市，滨海县人民医院呼吸与危重症医学科 2.224500江苏省盐城市，滨海县人民医院检验科 3.224799江苏省南京市，东南大学附属中大医院呼吸与危重症医学科 通信作者：薛方苏，E-mail：875910254@qq.com

Units：

1.Department of Respiratory and Critical Care Medicine, the People's Hospital of Binhai County, Yancheng 224500, China 2.Department of Clinical Laboratory, the People's Hospital of Binhai County, Yancheng 224500, China 3.Department of Respiratory and Critical Care Medicine, Zhongda Hospital, Southeast University, Nanjing 224799, China Corresponding author: XUE Fangsu, E-mail: 875910254@qq.com

关键词：

支气管炎; 非哮喘性嗜酸粒细胞性支气管炎; 白介素25; 嗜酸粒细胞;

Keywords：

Bronchitis; Non-asthmatic eosinophilic bronchitis; Interleukin 25; Eosinophils

CLC：

DOI：

10.12114/j.issn.1008-5971.2022.00.069

Funds：

盐城市科技发展计划项目（YK2020082）

摘要：

背景白介素(IL)-25可活化嗜酸粒细胞(EOS),能诱导与2型免疫相关的细胞因子的表达。研究发现,IL-25可以诱导气道高反应性,促进外周血和气道EOS浸润。但IL-25与非哮喘性嗜酸粒细胞性支气管炎(NAEB)的关系尚缺乏相关研究。目的 分析NAEB患者外周血、痰液IL-25水平及其临床意义。方法 选取2020年1月至2021年5月在滨海县人民医院诊断为NAEB的患者36例为NAEB组,同期选取于本院体检的健康者32例为对照组。比较两组受试者性别、年龄、EOS、呼出气一氧化氮(FeNO)、第1秒用力呼气容积(FEV1)及外周血、痰液IL-25水平,分析NAEB患者外周血、痰液IL-25水平与EOS、FEV1的相关性,绘制外周血、痰液IL-25水平诊断NAEB的ROC曲线。结果 NAEB组患者EOS、FeNO高于对照组,FEV1低于对照组(P<0.05)。NAEB组患者外周血、痰液IL-25水平高于对照组(P<0.05)。NAEB患者外周血、痰液IL-25水平与EOS均呈正相关(r值分别为0.632、0.372,P值分别为<0.001、0.025),与FEV1均呈负相关(r值分别为-0.543、-0.539,P值均为0.001)。ROC曲线分析结果显示,外周血IL-25水平诊断NAEB的曲线下面积(AUC)为0.982,最佳截断值为49.9 ng/L,灵敏度为0.916,特异度为0.875。痰液IL-25水平诊断NAEB的AUC为0.950,最佳截断值为46.4 ng/L,灵敏度为0.899,特异度为0.903。结论 NAEB患者外周血、痰液IL-25水平均升高,其与EOS呈正相关,与FEV1呈负相关,且对NAEB有一定诊断价值。

Abstract：

【Abstract】　Background Interleukin (IL) -25 can activate eosinophils (EOS) and induce the expression of cytokinesrelated to type 2 immunity. Studies have found that IL-25 can induce airway hyperresponsiveness and promote the infiltration ofEOS in peripheral blood and airway. However, the relationship between IL-25 and non-asthmatic eosinophilic bronchitis (NAEB)is still lack of relevant research. Objective To analyze IL-25 level in peripheral blood and sputum and its clinical significance inpatients with NAEB. Methods A total of 36 patients with NAEB admitted to the People's Hospital of Binhai County from January2020 to May 2021 were selected as the NAEB group, and 32 health people who underwent physical examination in the samehospital were selected as the control group. Gender, age, EOS, frectional exhaled nitric oxide (FeNO) , forced expiratory volume inone second (FEV1) , IL-25 level in peripheral blood and sputum were compared between the two groups. The correlation betweenIL-25 level in peripheral blood and sputum and EOS, FEV1 was analyzed. The ROC curve of IL-25 for the diagnosis of NAEB wasdrawn.Results EOS and FeNO of NAEB group were higher than those of control group (P < 0.05) . IL-25 level in peripheralblood and sputum of NAEB group was higher than that of control group (P < 0.05) . IL-25 level in peripheral blood and sputum ofNAEB patients was positively correlated with EOS (r values were 0.632, 0.372, P values were < 0.001, 0.025, respectively) , andit was negatively correlated with FEV1 (r values were -0.543, -0.539, both P values were 0.001) . The ROC curve analysis resultshowed that the area under curve (AUC) of IL-25 level in peripheral blood for diagnosing NAEB was 0.982. The best cut off valuewas 49.9 ng/L. Sensitivity was 0.916. Specificity was 0.875. The AUC of IL-25 level in sputum for diagnosing NAEB was 0.950.The best cut off value was 46.4 ng/L. Sensitivity was 0.899. Specificity was 0.903. Conclusion The level of IL-25 in peripheralblood and sputum in patients with NAEB increases, it is positively correlated with EOS, and negatively correlated with FEV1. Andit has certain diagnostic value for NAEB.

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