作者：王永兵，奚峰，蒋晓真，张丽娅，刘慧琴，杨娟，李刚，赵晓晖

单位：

201200 上海市浦东新区人民医院；通信作者：赵晓晖，E-mail：zhaoxh99990@sina.com

关键词：

帕金森病；益生菌；抗帕颗粒；1- 甲基 -4- 苯基 -1，2，3，6- 四氢吡啶；小鼠；动物行为学；干预性研究

Keywords：

Parkinson disease；Probiotics；Anti-parkinson potion；MPTP；Mice；Ethology；Intervention studies

CLC：

R 742.5

DOI：

DOI：10.3969/j.issn.1008-5971.2020.04.015

Funds：

上海市浦东新区医学重点学科建设项目（PWZxk207-11）；上海市浦东新区科经委民生基金项目（PKJ2017-Y32）

摘要：

背景　肠道菌群在帕金森病（PD）发生、发展过程中具有重要作用，而益生菌、抗帕颗粒的有效成分可通过调节肠道微生物成分、肠道炎性反应而影响肠道神经系统，并有可能通过脑肠轴神经传递而改善包括便秘在内的 PD 症状，延缓病情进展。目的　比较益生菌、抗帕颗粒对 PD 模型小鼠行为学的影响。方法　2018 年 9 月—2019年 10 月完成实验。选取雄性 SPF 级健康 C57BL/6 小鼠 111 只，随机分为 A 组（正常对照，n=20）、B1 组（PD 模型对照，n=20）、B2 组（PD 模型并益生菌干预，n=20）、B3 组（PD 模型并抗帕颗粒干预，n=20），B4 组（PD 模型并益生菌联合抗帕颗粒干预，n=20），五组均持续喂养 4 个月。比较五组小鼠喂养后 15 d 及 1、2、3、4 个月爬杆实验分值、悬挂实验分值、游泳实验分值。结果　时间与方法在爬杆实验分值上存在交互作用（P<0.05）；方法在爬杆实验分值上主效应显著（P<0.05）；时间在爬杆实验分值上主效应不显著（P>0.05）。B1 组、B2 组各时间，B3 组喂养 15 d 及 1、4 个月后，B4 组喂养 15 d 及 1、3、4 个月后爬杆实验分值低于 A 组（P<0.05）；B2 组喂养 3、4 个月后，B3 组、B4 组喂养 2、3、4 个月后爬杆实验分值高于 B1 组（P<0.05）；B3、B4 组喂养 2、4 个月后爬杆实验分值高于B2 组（P<0.05）。时间与方法在悬挂实验分值上存在交互作用（P<0.05）；时间、方法在悬挂实验分值上主效应显著（P<0.05）。B1 组各时间点，B2 组喂养 15 d 及 1、2、3 个月后，B3 组喂养 15 d 及 1、2、4 个月，B4 组喂养 15 d、1个月后悬挂实验分值低于A组（P<0.05）；B2组、B3组、B4组喂养2、3、4个月后悬挂实验分值高于B1组（P<0.05）；B3 组喂养 3 个月后、B4 组喂养 2 个月后悬挂实验分值高于 B2 组（P<0.05）；B4 组喂养 2 个月后悬挂实验分值高于B3组（P<0.05）。时间与方法在游泳实验分值上存在交互作用（P<0.05）；方法在游泳实验分值上主效应显著（P<0.05）；时间在爬杆实验分值上主效应不显著（P>0.05）。B1、B2 组各时间点，B3 组、B4 组喂养 15 d 及 1、2 个月后游泳实验分值低于 A 组（P<0.05）；B3、B4 组喂养 3、4 个月后游泳实验分值高于 B1 组（P<0.05）；B3、B4 组喂养 3 个月后游泳实验分值高于 B2 组（P<0.05）。结论　益生菌、抗帕颗粒对 PD 模型小鼠行为学均具有良好的改善作用，且抗帕颗粒较益生菌有一定的优势，而二者联合并未有明显优势。

Abstract：

Background　Intestinal flora plays an important role in the occurrence and development of Parkinson'sdisease（PD）.Probiotics and the effective components of anti-parkinson potion respectively affect the intestinal nerve system byregulating the composition of gut microbes and intestinal inflammatory response.They may improve the symptoms of PD includingconstipation，and delay the progress of the disease through neurotransmission of brain-gut axis.Objective　To compare theeffect of probiotics and anti-parkinson potion on the behavior of PD model mice.Methods　The experiment was completed fromSeptember 2018 to October 2019.111 healthy SPF male C57BL/6 mice were selected and randomly divided into group A（normalcontrol group，n=20），group B1（PD model control group，n=20），group B2（PD model with probiotic interventiongroup，n=20），group B3（PD model with anti-parkinson potion intervention group，n=20） and group B4（PD model withprobiotics combined with anti-parkinson potion intervention group，n=20），the five groups were feed continuously for 4 months.The scores of ploe test，traction test and swimming test 15 d，1 month，2 months，3 months and 4 months after feeding werecompared in the five groups.Results　There was significant interaction in ploe test score between time and method（P<0.05）；themain effects of method on ploe test score were significant（P<0.05）；the main effect of time on ploe test score was not significant（P>0.05）.Ploe test scores at all time points in group B1 and group B2，15 d，1 month，4 months after feeding in group B3，15 d，1 month，3 and 4 months after feeding in group B4 were lower than those in group A，respectively（P<0.05）；ploe testscores 3，4 months after feeding in group B2，2，3，4 months after feeding in group B3 and group B4 were higher than thosein group B1，respectively（P<0.05）；ploe test scores 2，4 months after feeding in group B3 and group B4 were higher thanthose in group B2，respectively（P<0.05）.There was significant interaction in traction test score between time and method（P<0.05）；the main effects of time and method on traction test score were significant，respectively（P<0.05）.Tractiontest scores at all time points in group B1，15 d and 1 month，2，3 months after feeding in group B2，15 d and 1 month，2，4 months after feeding in group B3，15 d and 1 month after feeding in group B4 were lower than those in group A，respectively（P<0.05）；traction test scores 2，3，4 months after feeding in group B2，group B3 and group B4 were higher than those ingroup B1，respectively（P<0.05）；traction test scores 3 months after feeding in group B3，2 months after feeding in group B4were higher than those in group B2，respectively（P<0.05）；traction test score 2 months after feeding in group B4 was higherthan that in group B3（P<0.05）.There was significant interaction in swimming test score between time and method（P<0.05）；the main effects of method on swimming test score was significant（P<0.05）；the main effect of time on swimming test scorewas not significant（P>0.05）.Swimming test scores at all time points in group B1 and group B2，15 d and 1 month，2 monthsafter feeding in group B3 and group B4 were lower than those in group A，respectively（P<0.05）；swimming test scores 3，4 months after feeding in group B3 and group B4 were higher than those in group B1，respectively（P<0.05）；swimmingtest scores 3 months after feeding in group B3 and group B4 were higher than those in group B2，respectively（P<0.05）.Conclusion　Probiotics and anti-parkinson potion can improve the behavior of PD model mice，and anti-parkinson potion hascertain advantages over probiotics，but the combination of them do not have obvious advantages.

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