作者：郭荷娜1 ，康蓓 1 ，赵婧钰 2 ，杨谦 1

单位：

1.710068 陕西省西安市，陕西省人民医院神经内二科；2.710068 陕西省西安市，西安医学院；通信作者：郭荷娜，E-mail：zihe821201@163.com

Units：

1.Department of Neurology，Shaanxi Provincial People's Hospital，Xi'an 710068，China；2.Xi'an Medical University，Xi'an 710068，China；Corresponding author：GUO Hena，E-mail：zihe821201@163.com

关键词：

缺血再灌注损伤；脑缺血；丹红注射液；新生微血管；侧支循环

Keywords：

Ischemia-reperfusion injury；Brain ischemia；Danhong injection；Neovascularization；CollateralCirculation

CLC：

R 619.9

DOI：

DOI：10.3969/j.issn.1008-5971.2020.02.011

Funds：

陕西省重点研发计划项目（2017SF-096）

摘要：

背景　新生血管形成是最终的侧支代偿途径，探究丹红注射液对新生血管的影响对其作用机制研究具有一定价值。目的　探究丹红注射液对脑缺血再灌注损伤大鼠皮质区新生微血管的影响。方法　2018 年 12 月—2019年 6 月，将 84 只雄性 SD 大鼠随机分为假手术组（n=12）、造模组（n=36）及丹红组（n=36）。参照改良 LONGA线栓法制备脑缺血再灌注损伤大鼠模型，其中造模组和丹红组大鼠制备脑缺血再灌注损伤模型，假手术组大鼠不插线栓；丹红组大鼠于拔出线栓后腹腔注射丹红注射液，造模组和假手术组大鼠均于相同时间点腹腔注射等量 0.9% 氯化钠溶液。比较三组大鼠造模后 24 h、72 h、7 d 神经行为学评分、脑梗死体积及脑缺血再灌注皮质区新生微血管数目。结果　（1）假手术组大鼠造模后 24 h、72 h 及 7 d 神经行为学评分均为 0。造模组和丹红组大鼠造模后 24 h、72 h 神经行为学评分比较，差异无统计学意义（P>0.05）；丹红组大鼠造模后 7 d 神经行为学评分低于造模组（P<0.05）。（2）假手术组大鼠造模后 24 h、72 h 及 7 d 脑梗死体积均为 0。丹红组大鼠造模后 24 h、72 h 及 7 d 脑梗死体积小于造模组（P<0.05）。（3）造模组和丹红组大鼠造模后 24 h 脑缺血再灌注皮质区新生微血管数目多于假手术组（P<0.05）；造模组和丹红组大鼠造模后 72 h 及 7 d 脑缺血再灌注皮质区新生微血管数目多于假手术组，丹红组大鼠脑缺血再灌注皮质区新生微血管数目多于造模组（P<0.05）。结论　丹红注射液可有效增加脑缺血再灌注损伤大鼠皮质区新生微血管数量，改善脑侧支循环，进而减轻神经功能缺损、缩小脑梗死体积。

Abstract：

Background　Neovascularization is the ultimate way of collateral compensation，thus it is with referencevalue to explore the impact and action mechanism of Danhong injection on neovascularization. Objective　To investigate theimpact of Danhong injection on neovascularization in cortex in rats with cerebral ischemia-reperfusion injury. Methods　FromDecember 2018 to June 2019，a total of 84 male SD rats were randomly divided into sham-operation group（n=12），modelgroup（n=36） and Danhong group（n=36）. Model of cerebral ischemia-reperfusion injury was established by modifiedLONGA suture-occluded method，thereinto rats in model group and Danhong group were prepared for model of cerebralischemia-reperfusion injury，while rats in sham-operation group did not receive suture-occlusion；rats in Danhong groupreceived intraperitoneal injection of Danhong injection after suture-occlusion，while rats in model group and sham-operationgroup received intraperitoneal injection of same amount of 0.9% sodium chloride solution after suture-occlusion. Neurobehavioralscore，volume of cerebral infarction and number of neovascularization in cerebral ischemia-reperfusion cortex were compared inthe three groups 24 hours，72 hours and 7 days after modeling. Results　（1）Neurobehavioral score in sham-operation groupwere all 0 24 hours，72 hours and 7 days after modeling. There was no statistically significant difference in neurobehavioral scorebetween model group and Danhong group 24 or 72 hours after modeling（P>0.05），while neurobehavioral score in Danhonggroup was statistically significantly lower than that in model group 7 days after modeling（P<0.05）.（2）Volume of cerebralinfarction in sham-operation group were all 0 24 hours，72 hours and 7 days after modeling. Volume of cerebral infarction inDanhong group was statistically significantly smaller than that in model group 24 hours，72 hours and 7 days after modeling，respectively（P<0.05).（3）Number of neovascularization in cerebral ischemia-reperfusion cortex in model group and Danhonggroup was statistically significantly more than that in sham-operation group 24 hours after modeling，respectively（P<0.05）；number of neovascularization in cerebral ischemia-reperfusion cortex in model group and Danhong group was statisticallysignificantly more than that in sham-operation group 72 hours and 7 days after modeling，respectively，meanwhile number ofneovascularization in cerebral ischemia-reperfusion cortex in Danhong group was statistically significantly more than that in modelgroup，respectively（P<0.05）. Conclusion　Danhong injection can effectively increase the number of neovascularizationin cortex in rats with cerebral ischemia-reperfusion injury，improve the collateral circulation of brain，and then reduce theneurological deficit and volume of cerebral infarction.

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